caco2 intestinal epithelial cell lines  (ATCC)

Journal: mSphere

Article Title: Evidence for a Causal Role for Escherichia coli Strains Identified as Adherent-Invasive (AIEC) in Intestinal Inflammation

doi: 10.1128/msphere.00478-22

Figure Lengend Snippet: E. coli strains vary widely in their ability to invade and replicate in epithelial cells but not in adherence to epithelial cells. Caco2 cells were infected with E. coli strains at an MOI of 10 for 3 h. (A) Adherence of E. coli strains to the Caco2 monolayer. (B) Invasion of Caco2 cells by E. coli strains. The percentage of intracellular bacteria at 1 h after gentamicin treatment was calculated relative to that of the original inoculum. (C) Survival and replication of E. coli strains within Caco2 cells at 24 h postinfection. The percentage of intracellular bacteria at 24 h postinfection was calculated relative to that obtained at 1 h after gentamicin treatment (defined as 100% and shown as a dashed line). Values were log 10 transformed. For all panels, data represent the mean of triplicate technical replicates ± SEM from at least three independent experiments.

Article Snippet: The Caco2 intestinal epithelial cell line (ATCC HTB-37) was used to assess E. coli adherence to, invasion of, and survival in intestinal epithelial cells.

Techniques: Infection, Bacteria, Transformation Assay

Journal: mSphere

Article Title: Evidence for a Causal Role for Escherichia coli Strains Identified as Adherent-Invasive (AIEC) in Intestinal Inflammation

doi: 10.1128/msphere.00478-22

Figure Lengend Snippet: E. coli strains assigned an in vitro AIEC phenotype exacerbate intestinal inflammation in mice. (A) Macroscopic cecal lesion scores ( n ≥ 10 mice per treatment). (B) Microscopic cecal lesion scores ( n ≥ 5 mice per treatment). (C) Representative photomicrographs of hematoxylin-and-eosin-stained cecal tissues taken at ×20 objective magnification. (D) Diagram showing the distribution of disease-causing E. coli strains across different phylogroups. Data were analyzed using a nonparametric Kruskal-Wallis test followed by an unpaired Mann-Whitney post hoc test. Data represent the mean ± SEM. Red dots (A and B) and type (C) represent E. coli strains that replicated in J774 macrophages and invaded and replicated in Caco2 epithelial cells. Green dots (A and B) and type (C) represent strains that failed to replicate in macrophages and invade/replicate in epithelial cells. Black dots (A and B) and type (C) represent strains were either able to invade epithelial cells but not replicate in macrophages or vice versa. Treatments with unique letters (either a, b, c, d, or e) or combinations of letters are significantly different from one another at a P of <0.05.

Article Snippet: The Caco2 intestinal epithelial cell line (ATCC HTB-37) was used to assess E. coli adherence to, invasion of, and survival in intestinal epithelial cells.

Techniques: In Vitro, Staining, MANN-WHITNEY

Journal: Pathogens

Article Title: Role of the β-Catenin/REG Iα Axis in the Proliferation of Sessile Serrated Adenoma/Polyps Associated with Fusobacterium nucleatum

doi: 10.3390/pathogens10040434

Figure Lengend Snippet: Effect of Wnt agonist SKL2001 on nuclear β-catenin translocation and REG Iα expression in Caco2 cells. ( A ) Representative photograph showing nuclear β-catenin (red) translocation in Caco2 cells treated with Wnt agonist SKL2001. Caco2 cells (5 × 10 4 ) were cultured in 12-well plates for 24 h and then treated with Wnt agonist SKL2001 (40 mM) for 24 h. After stimulation, the cells were fixed with methanol for 10 min at room temperature, incubated with primary anti-β-catenin antibody for 1 h, followed by TRITC-labeled secondary anti-rabbit antibodies for 1 h, nuclear counterstained using Antifade Mountant with DAPI (blue) and observed under Olympus BX53 microscope. Bar = 20 mm. ( B ) Change in REG Iα expression in in Caco2 cells treated with Wnt agonist SKL2001. Results are expressed as the mean ±SE. * p < 0.05 vs. control group.

Article Snippet: Human intestinal epithelial cell line Caco2 was obtained from ATCC (Manassas, VA, USA) and cultured in RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA) with 10% fetal bovine serum (Biowest, Nuaillé, France) in a humidified incubator at 37 °C with an atmosphere of 5% CO2.

Techniques: Translocation Assay, Expressing, Cell Culture, Incubation, Labeling, Microscopy, Control